Development and validation of a Reverse-phase Liquid Chromatographic method for simultaneous determination of timolol maleate and Human serum albumin in albumin nanoparticles

C. BOIERO; M. LONGHI; D. ALLEMANDI; J. LLABOT

Pamplona

Congreso; 19° Simposio Internacional de Microencapsulacion; 2013

International Microencapsulation Society

Topical application onto the eye´s surface is a common route of drugs administration; however the physiology of the eye itself makes  difficult to achieve effective concentrations of drug for extended periods of time. Nanoparticles (Nps) have emerged as a suitable vehicle for the administration of drugs due to in many cases a significant increase of drug bioavailability was observed.  Macromolecular substances can be used in the preparation of Nps, wherein human serum albumin (HSA) emerges as a suitable material (nontoxic, non-immunogenic, biocompatible and biodegradable). Our work focuses on the production of HSA-Np for the topical administration of Timolol Maleate (TM) for the treatment of glaucoma. One of the most important parameters to characterize these Nps is to evaluate the encapsulation yield and process performance besides the physico-chemical characteristics (particle size, polydispersity index, Z potential, yield, and morphology). For this reason we have developed a one step HPLC method, which is simple, rapid and reproducible for the simultaneous determination of HSA and TM.