Caracterización biológica de composites de Titanio-Hidroxiapatita sinterizados a baja temperatura

COMÍN R; CID MP; GRINSCHPUN L; OLDANI C; TABORDA R; SALVATIERRA NA

Congreso; XX Congreso de Bioingeniería - IX Jornada de Ingeniería Clínica; 2015

Bone tissue loss caused by severe injury or disease is a critical problem in orthopedic clinics, andinvolves bone repairs and substitutes. At present, several devices and biomaterials in the treatment of thesedefectsare used. Increased interest in the use of titanium and hydroxyapatitecomposites for biomedicalapplications has been observed in recent years due to a good combination of the hydroxyapatite bioactivity andthe favorable mechanical properties of metals. Powder metallurgyis a method that uses powder from titaniumand hydroxyapatite to obtain composites having hydroxyapatite phases in metallic matrix. One of the steps ofthis methodincludes a sintering stage ranged 1000-1300ºC. However, in this system the presence of titaniumaccelerated dehydroxylation and the decomposition of hydroxyapatite to form tetracalcium phosphate andcalcium oxideand these were detected at a temperature as low as 800°C. This imposes certain limitations on thethermal conditions for the manufacture of composites from a mixture of titanium and hydroxyapatite. In thiswork, we obtained a titanium-hydroxyapatite composite sintered at 800°C and evaluated its invitrobiocompatibility. The MTT assay was carried out to assess cytotoxicity on Vero and NIH 3T3 cell lines inaccording to ISO 10993 Standard. In addition, the cell morphology and adhesion on composite surfacewereanalyzed usingfluorescence and SEM microscopy. Our data show that the extracts from sintered compositeat 800°C did not produce toxicity on Vero or NIH 3T3 cells neither affected the morphology and density of cellsgrown. Finally, the NIH 3T3 cells were adhered and proliferated on entire surface covering titaniumhydroxyapatitecomposite at day 4 of cell culture. These results provide in vitro evidence of goodbiocompatibility and bioactivity of this titanium-hydroxyapatitecomposite sintered at low temperature.