INVESTIGADORES
TORRES Sebastian
congresos y reuniones científicas
Título:
Production and purification of a solvent resistant esterase from B. licheniformis S-86
Autor/es:
TORRES SEBASTIÁN; BAIGORÍ MARIO; PANDEY ASHOK; CASTRO GUILLERMO R.
Lugar:
Trivandrum, India
Reunión:
Conferencia; International Conference on New Horizons in Biotechnology (NHBT - 2007); 2007
Institución organizadora:
National Institute for Interdisciplinary Science & Technology (NIIST)
Resumen:
Esterases have attracted great interest due to their ability to synthesize new compounds of high commercial value. Wild-type B. licheniformis S-86 produces esterases stable and active in organic solvents. The aim of this work was to study the production and purification of type II esterase. Esterase production was carried out in a 400 ml-airlift reactor type at 50ºC, supplemented with oils and surfactants and was purified to homogeneity by (NH4)2SO4 precipitation, ultrafiltration, and thermal treatment, followed by hydrophobic interaction and ion-exchange chromatography. Grape oil and tween 20 to 60 enhance the enzyme production between 1.4 to 2.2 folds. Type II esterase was purified 76.7-fold with a specific activity of 135 U/mg. The molecular mass of the enzyme was estimated to be 38 kDa. This enzyme was active mostly on esters with short acyl chains with a Km of 80.2 mM and a Vmax of 256.4 µmol/min.mg for p-nitrophenylacetate.