INVESTIGADORES
RAPISARDA Viviana Andrea
congresos y reuniones científicas
Título:
PROTEOMIC ANALYSIS OF LACTOBACILLUS PARAPLANTARUM CRL 1905 IN RESPONSE TO DIFFERENT PHOSPHATE CONCENTRATIONS
Autor/es:
ARAOZ, M.; GRILLO-PUERTAS, M.; HEBERT, E. M.; VILLEGAS, J. M.; RAPISARDA, V. A.
Lugar:
Salta
Reunión:
Congreso; Joint LV Annual SAIB Meeting and XIV PABMB Congress; 2019
Institución organizadora:
SAIB
Resumen:
Polyphosphate (polyP) is a linear polymer that plays important roles in microbial physiology. The metabolism of this polymer has been scarcely explored in lactic bacteria, microorganisms of great interest in the food industry and in human and animal health. Our previous results evidenced the ability of Lactobacillus paraplantarum CRL 1905 to maintain high levels of polyP in stationary phase in a high phosphate (Pi) medium (CDM+P). In this condition, cells increased their survival and tolerance to acidity and bile salts, compared to those grown in sufficient Pi medium (CDM-P). The aim of this work was to evaluate the influence of environmental Pi concentration in the proteomic profile of CRL 1905 strain. The comparative proteomic analysis was carried out in cells grown in CDM+P or CDM-P during 48 h. From the 51 proteins that were differentially expressed, 29 were up-regulated and 22 were down-regulated in cells grown in CDM+P vs CDM-P. PdhA, PdhB, PdhC, Pox3, Ack2, PflB, GlpK1, Xfp, RpiA, GalM3, RbsK, and Pgm1 proteins, involved in glycolysis, pyruvate metabolism and pentose phosphate pathway, were up-regulated in high Pi stationary phase cells, implying an active metabolism similar to that of exponential phase cells. Upp, PyrB, and PyrR, proteins involved in nucleotide transport and metabolism, were also up-regulated in the high Pi condition. On the other hand, chaperones implicated in stress response (Hsp1, Hsp3, and ClpX) were repressed in CDM+P, inferring that high polyP levels in stationary phase could act as an inorganic chaperone to maintain protein stability. Enzymes involved in cell surface modification (Lp_3421, Acm2, MltG, Alr, and FmlA) were also down-regulated in CDM+P. In accordance with the proteomic analysis, biofilm formation capacity of CRL 1905 increased in CDM-P, being ~ 3 times higher than that produced in CDM+P. Together, our results indicate that L. paraplantarum CRL 1905 grown in high Pi conditions presented an advantageous cellular fitness during the stationary phase by modifying different metabolic pathways.