INVESTIGADORES
RAPISARDA Viviana Andrea
congresos y reuniones científicas
Título:
Effect of phosphate on different virulence traits of uropathogenic E. coli isolated from prostatitis
Autor/es:
GRILLO-PUERTAS, M.; RINTOUL, M. R.; SOTO, S. M.; RAPISARDA, V. A.
Lugar:
Mendoza
Reunión:
Congreso; XLVIII Annual Meeting of SAIB (Argentine Society for Biochemistry and Molecular Biology); 2012
Resumen:
Uropathogenic E. coli (UPEC) strains, the most frequent cause of urinary tract infections, has several virulence factors to colonize host tissues. Biofilm formation allows cells to persist a long time in the genitourinary tract and interfere with bacterial eradication. We have shown that laboratory E. coli cells grown in media containing phosphate (Pi) concentrations ≥35 mM were defective in biofilm formation. The aim of this work was to determine a possible Pi-dependent effect on biofilm formation, production of attachment factors and antibiotic resistance in E. coli prostatitis isolates. A total of 58 strains collected from patients were analyzed for in vitro biofilm formation by the O?Toole and Kolter method technique and confocal microscopy. Curli and cellulose production was evaluated with Congo Red and Calcofluor assays, respectively. 65.5% of the isolates were considered positive for in vitro biofilm formation, but different behaviors were observed in respect to the media phosphate concentration. MIC for nalidixic acid and ciprofloxacin was determined in planktonic and static cultures changing Pi concentrations. When antibiotics were added after 24 h over a preformed biofilm, no sensitivity was observed. This work is a first approach that shows how environmental Pi concentrations could modulates different phenotypes related with UPEC virulence.