INVESTIGADORES
MOZZI Fernanda Beatriz
congresos y reuniones científicas
Título:
Adhesion of Streptococcus thermophilus CRL 1190 to the gastruc mucosa in mice detected by scanning electron microscopy
Autor/es:
MARCIAL G.E.; RODRÍGUEZ M.C.; MÉDICI M.; MOZZI F. ; FONT DE VALDEZ G.
Lugar:
Egmond aan Zee
Reunión:
Simposio; LAB 10th Symposium; 2011
Resumen:
Fermented
milk with Streptococcus thermophillus CRL
1190 (FM-1190) has been tested in an in
vivo model of active and superficial chronic gastritis induced by
acetylsalicylic acid (ASP) in BALB/c mice.
Therapeutic and
preventive treatments with FM-1190 reduced the lesion on gastric mucosa after 10-d
treatment. Histological slides of the treated animals were similar to control mice
(without gastritis induction); the former group also displayed increase in IL-10,
decrease in TNF-α, and a greater production of mucus. These effects were
associated to the exo-polysaccharide (EPS-1190) produced by the strain. Interactions
of S. thermophillus CRL 1190 with the
gastric mucosal epithelial was addressed in this study. Balb/C mice received FM-1190
during 7d (preventive treatment) followed by 10d ASP administration. Control
groups received different treatments: A)- the strain CRL 1190 (without EPS)
suspended in milk ; B)- similar to A but the cells suspended in phosphate-buffer
saline (PBS). A third group (group C) received no treatment. All groups had
free access to conventional balanced diet and water ad libitum. Scanning
electron microscopy revealed that the strain CRL 1190 remained attached to the
gastric mucosal 10d after ending the treatment (FM-1190 feeding). In contrast,
no bacterial cells were observed in the gastric mucosal in any of the control
groups (A, B or C). The hypothesis is, that EPS-1190 together with milk
proteins creates a microenvironment that allows the probiotic S. thermophillus CRL 1190 to attach to
the gastric mucus and to resist under the stress conditions of the gastric
environment. Studies are being conducted to confirm this statement.