Cloning of BoMyr from broccoli. Analysis of its expression during senescence and the effect of plant regulators

GOMEZ LOBATO MARÍA EUGENIA; CIVELLO PEDRO; MARTINEZ GUSTAVO

Puerto Madryn

Congreso; XLVI Reunión Anual de SAIB (Sociedad Argentina de Investigación en Bioquímica y Biología Molecular); 2010

SAIB

Broccoli (Brassica oleracea) is a vegetable belonging to the family of Cruciferae. It is harvested when their inflorescences are still immature, which causes high stress and triggers the senescence. Fresh broccoli contains glucosinolates as a secondary metabolites. There is strong evidence that consumption of brassica vegetables is associated with a decreased risk for several cancers, probably to their glucosinolate content. Glucosinolates co-exist with myrosinases, which are responsible for glucosinolate turn-over. When tissue is damaged, glucosinolates are hydrolyzed by myrosinases into different degradation products which play roles in plant interaction with pathogens. In this work, we cloned a fragment of Myrosinase (Myr) from broccoli and analyzed its expression by qPCR during postharvest senescence and under the effect of different plant regulators. Broccolis were treated with cytokinin and ethylene, in order to delay or accelerate senescence, respectively. It was observed that, the expression of BoMyr was higher in the inflorescences compared with the stems and leaves. The expression decreased during the senescence and it was modulated by the hormone treatments. It was observed that the expression decreased with ethylene treatment, but the decrement was delayed in samples treated with cytokinin. In this last treatment, the expression after 24 h had similar values to initials.