INVESTIGADORES
GOLDBAUM Fernando Alberto
artículos
Título:
Llama single domain antibodies as a tool for molecular mimicry
Autor/es:
ZAREBSKI, L; URRUTIA, M; GOLDBAUM, FA
Revista:
JOURNAL OF MOLECULAR BIOLOGY
Referencias:
Año: 2005 vol. 349 p. 814 - 824
ISSN:
0022-2836
Resumen:
In camelids, a subset of the immunoglobulins consists of heavy-chain
homodimers devoid of light chains, and are thus called heavy-chain IgGs
(hcIgGs). Their variable region (VHH) is the smallest antigen-binding
fragment possible, and being just one polypeptide chain it is especially
suitable for engineering. In particular, camelid single domain antibodies
might be very useful for molecular mimicry and anti-idiotypic vaccination.
In the present work, we show that llamas immunized with an anti-DNA
mouse mAb develop an important anti-Id response. Selection of VHHs by
phage display, with specific elution of bound phages with the external
antigenic DNA, shows that selected private anti-Id VHHs compete for
binding to the external antigen and bear a functional mimicry of the DNA.
These results indicate that llama anti-Id single domain antibodies would be
an excellent tool for molecular mimicry studies.