Brucella spp. lumazine synthase: a novel antigen delivery system

SCIUTTO, E; TOLEDO, A; CRUZ, C; ROSAS, G; MENESES, G; LAPLAGNE, DA; AINCIART, N; CERVANTES, J; FRAGOSO, G; GOLDBAUM, FA

VACCINE

Año: 2005 vol. 23 p. 2784 - 2790

0264-410X

Lumazine synthase from Brucella spp. (BLS) was evaluated as a protein carrier to improve antigen delivery of KETc1, one of the peptides of the anti-cysticercosis vaccine. KETc1 becomes antigenic, preserved its immunogenicity and its protective capacity when expressed as a recombinant chimeric protein using Brucella spp. lumazine synthase. KETc1 and BLS-KETc1 were not MHC H-2d, H-2k nor H-2b haplotyperestricted albeit KETc1 is preferentially presented in the H-2b haplotype. These findings support that BLS is a potent new delivery system for the improvement of subunit vaccines.Brucella spp. (BLS) was evaluated as a protein carrier to improve antigen delivery of KETc1, one of the peptides of the anti-cysticercosis vaccine. KETc1 becomes antigenic, preserved its immunogenicity and its protective capacity when expressed as a recombinant chimeric protein using Brucella spp. lumazine synthase. KETc1 and BLS-KETc1 were not MHC H-2d, H-2k nor H-2b haplotyperestricted albeit KETc1 is preferentially presented in the H-2b haplotype. These findings support that BLS is a potent new delivery system for the improvement of subunit vaccines.Brucella spp. lumazine synthase. KETc1 and BLS-KETc1 were not MHC H-2d, H-2k nor H-2b haplotyperestricted albeit KETc1 is preferentially presented in the H-2b haplotype. These findings support that BLS is a potent new delivery system for the improvement of subunit vaccines.H-2b haplotype. These findings support that BLS is a potent new delivery system for the improvement of subunit vaccines.