NATURAL ANTIFUNGAL EXTRACTED FROM PROPOLIS OF SANTIAGO DEL ESTERO, ARGENTINA.

DANERT C.; ZAMPINI I.C; ORDOñEZ R.M; ALBERTO M. R.; MALDONADO L.; BEDASCARRASBURE E.; ISLA M.I

Congreso; CONGRESO INTERNACIONAL DE APICULTURA DE APIMONDIA.; 2011

The Argentine Northwest is a region with great potential for production of bee products such as honey and propolis. Functional diversity of propolis is influenced by the chemical variability of this complex resinous material and this in turn by their botanical origin. Hence, the importance of determine the chemical composition and bioactivity of the propolis from different regions of Argentina to ensure quality and improve competitiveness. In this context, the aim of this study was to determine the antifungal capacity of propolis from different zones of Santiago del Estero, Argentina. From five samples of raw propolis, extractive solutions highly polar, alcohol-free and standardized on their content of total phenolics and flavonoids were prepared. Qualitative microbiological tests were performed (bioautographyc assays) and quantitative (minimum inhibitory concentration, MIC) against various Candida species (Candida albicans, C. tropicalis, C. parasilopsis, C. glabrata and C. guilleirmondii). The values of total phenolic compounds concentration ranged from 178 to 442 ìg GAE/ml (micrograms of gallic acid equivalent/ml of extract) and flavonoids concentration between 424 to 865 ìg QE/ml (micrograms of quercetin equivalent/ml of extract). From all analyzed extracts only the corresponding to propolis from La Banda showed inhibitory activity on growth of different Candida species with MIC values between 200-400 mg DWE/ml (milligrams of dry weight equivalent/ml of extract). There was no correlation between antifungal activity and content of phenolic compounds and flavonoids in different samples. Our results indicate that extracts of propolis in this region could be used as a antifungal in topical applications.Candida albicans, C. tropicalis, C. parasilopsis, C. glabrata and C. guilleirmondii). The values of total phenolic compounds concentration ranged from 178 to 442 ìg GAE/ml (micrograms of gallic acid equivalent/ml of extract) and flavonoids concentration between 424 to 865 ìg QE/ml (micrograms of quercetin equivalent/ml of extract). From all analyzed extracts only the corresponding to propolis from La Banda showed inhibitory activity on growth of different Candida species with MIC values between 200-400 mg DWE/ml (milligrams of dry weight equivalent/ml of extract). There was no correlation between antifungal activity and content of phenolic compounds and flavonoids in different samples. Our results indicate that extracts of propolis in this region could be used as a antifungal in topical applications.