IBBM   21076
INSTITUTO DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
A deep rough type structure in Bordetella bronchiseptica lipopolysaccharide affects the host immune response.
Autor/es:
SISTI, FEDERICO; FERNANDEZ, JULIETA; HIGGINS, SARAH; MILLS, KINGSTON; HOZBOR, DANIELA FLAVIA
Lugar:
Baltimore Maryland
Reunión:
Simposio; 9no Simposio de Bordetella; 2010
Resumen:
It is well
known that Bordetella bronchiseptica lipopolysaccharide plays an essential role
in bacteria-host interaction. Using a defective mutant lacking O antigen with a
deep rough structure (named BbLP39), we have demonstrated the importance of the
complete LPS structure for effective colonization of mice with B. bronchiseptica. Moreover, we observed that
this mutant had diminished ability to
adhere to host cells. Here we showed that the deep rough mutant BbLP39 was able
to infect and persist in the lungs of TLR4-defective mice beyond 5 days post
infection. However, unlike the parental strain, which resulted in deaths in
100% of infected mice, infection with BbLP39 was not lethal, over a range of
dose used for the intranasal challenge..
However, the infection persisted in TLR4-defective mice following infection
with a range of doses of BbLP39. To test if our findings reflect difference in
the immune responses to the deep rough mutant compared with the parental
strain, we examined cytokine production in vivo and in vitro using different
forms of purified LPS. Despite the fact that the deep rough LPS has the same
lipid A structure of the parental LPS, the BbLP39 LPS induced significantly
higher TNF-a and IL-10 mRNA expression (3 and 7 times respectively) when
compared with controls. Experiments with
bone marrow dendritic cells showed that deep rough LPS is a potent activator of
IL-10 production. Our findings demonstrate for the first time differences in
host response due to distal LPS structures, supporting the hypothesis that
differences in LPS structure observed between strains may be influence the
interaction between B. bronchiseptica and its hosts.