IBBM 21076
INSTITUTO DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Unidad Ejecutora - UE
artículos
Título:
First genomic analysis of the broad-host-range Rhizobium sp. LPU83 strain, a member of the low-genetic diversity Oregon-like Rhizobium sp. group
Autor/es:
TORRES TEJERIZO, GONZALO; DEL PAPA, M. FLORENCIA; DRAGHI, WALTER; LOZANO, MAURICIO; GIUSTI, M. ANGELES; MARTINI, M. CARLA; SALAS, M. EUGENIA; SALTO, ILEANA; WIBBERG, DANIEL; SZCZEPANOWSKI, RAFAEL; WEIDNER, STEFAN; SCHLUTER, ANDREAS; LAGARES, ANTONIO; PISTORIO, MARIANO
Revista:
JOURNAL OF BIOTECHNOLOGY
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2011
ISSN:
0168-1656
Resumen:
Alfalfa (Medicago sativa) isthemostcultivatedforagelegumeforcattleandanimalfeeding,occupyingabout 32millionhectaresovertheworld.ManagementoftheN2-fixing symbiosisofthisplanttomaxi-mize cropproductionisthereforeanimportantobjective.Afundamentalconstrainttothisaimemergeswhen amoderatelylowsoilpHhamperstheestablishmentofaneffectivesymbiosiswithindigenousand/or inoculatedrhizobia.Besidestheassociationofalfalfawith Ensifer (Sinorhizobium) meliloti, thislegume isabletoestablishasymbiosiswith Ensifer (Sinorhizobium) medicae and withlesscharacterizedtypes ofrhizobia,suchastheOregon-likestrains, Rhizobium sp. Or191initiallyisolatedintheUSA,andtheRhizobium sp. LPU83strain,fromArgentina.Thesestrainsareacid-tolerant,highlycompetitiveforacidic-soil-alfalfa nodulation,butinefficientforbiologicalnitrogenfixationwithalfalfa.Thesefeaturespositionthe Oregon-likerhizobiaasstrainsofpotentialriskinagriculturalsoilscomparedwiththeefficientsym-biont E. meliloti. Moreover,thecollectedgeneticinformationhasrevealedthatthegenomicstructureofthese rhizobialisolatesiscomplexintermsofsequencesimilaritiessharedwithotherrhizobia.Sucha?patched? geneticcompositionhasobviouslyimposedsevererestrictionstotheclassicaltaxonomyofthese rhizobia.InthisworkwesummarizetheaccumulatedknowledgeabouttheOregon-likerhizobiaand presentaphylogeneticanalysisbasedongenomesequencedataof Rhizobium sp. LPU83obtainedbya high-throughputsequencingontheGenomeSequencerFLXTitaniumplatform.Theaccessibilityofthecomplete genomicsequencewillreleaseupmoreexperimentalpossibilitiessincethisinformationwillthen enablebiochemicalstudiesaswellasproteomicsandtranscriptomicsapproaches.
