Variation of cattle major histocompatibility complex (BoLA) DRB3 allele frequencies within different farm, breed and countries in South America.

TAKESHIMA S-N; MATSUMOTO Y.; ARAINGA-RAMIREZ M.; KIM J.; MIYASAKA T.; XUE G.; DE LA BARRA V.G.; GIOVAMBATTISTA G.; POFCHER E.; RIVERA-GERONIMO H.; SAITO H.; ACOSTA T.J.; KANEMAKI M.; ORTIZ M.L.; OLTRA J.; ONUMA M.; AIDA Y.

Edimburgo

Conferencia; XXXII Conference of the International Society for Animal Genetics; 2010

International Society for Animal Genetics

The cattle major histocompatibility complex (BoLA)-DRB3 gene is the most polymorphic class II locus in cattle, and these alleles are associated with susceptibility to several infectious diseases. To investigate BoLA-DRB3 allele of various cattle breeds in South American, we genotyped 2343 head of cattle containing 369 Japanese, 385 Argentina, 139 Paraguayan, 328 Peruvian, 499 Bolivian and 623 Chilean using new PCR-sequence based typing method. Collected breeds were Wagyu, Japanese Shorthorn, Jersey, Holstein, Gir, Zebu, Nelore, Ovelo Colorado, Ovelo Negro, Black and Red Angus, and mixed. 72 alleles out of known 118 alleles and three unknown new alleles are detected. All three new alleles were detected from Bolivian mix-breed and one of three was also detected from Peruvian and Paraguayan Holstein. Number of detected alleles in each breeds were from 14 (Japanese Jersey) to 35 (Peruvian Zebu and Bolivian Mix breed) and Expected Heterozygosity were from 0.858 (Peruvian Zebu) to 0.952 (Bolivian Mix breed). To overview of allele frequencies, we constructed phylogenetic tree which clearly showed that intrinsic allele frequencies were neither depending on countries nor on farm but instead on breed. Especially, Holstein has intrinsic allele frequencies in all farms except one in Argentine. Meanwhile, some Ovelo Colorado breed were very near to Holstein but from some other farm it is most far from Holstein within the Bos Taurus breed. Moreover, Japanese Wagyu and Chilean Wagyu are resembled but Chilean Wagyu also had the characteristic of Angus. These frequency data provide infrastructure for understating the host factor of disease susceptibility. DRB3 gene is the most polymorphic class II locus in cattle, and these alleles are associated with susceptibility to several infectious diseases. To investigate BoLA-DRB3 allele of various cattle breeds in South American, we genotyped 2343 head of cattle containing 369 Japanese, 385 Argentina, 139 Paraguayan, 328 Peruvian, 499 Bolivian and 623 Chilean using new PCR-sequence based typing method. Collected breeds were Wagyu, Japanese Shorthorn, Jersey, Holstein, Gir, Zebu, Nelore, Ovelo Colorado, Ovelo Negro, Black and Red Angus, and mixed. 72 alleles out of known 118 alleles and three unknown new alleles are detected. All three new alleles were detected from Bolivian mix-breed and one of three was also detected from Peruvian and Paraguayan Holstein. Number of detected alleles in each breeds were from 14 (Japanese Jersey) to 35 (Peruvian Zebu and Bolivian Mix breed) and Expected Heterozygosity were from 0.858 (Peruvian Zebu) to 0.952 (Bolivian Mix breed). To overview of allele frequencies, we constructed phylogenetic tree which clearly showed that intrinsic allele frequencies were neither depending on countries nor on farm but instead on breed. Especially, Holstein has intrinsic allele frequencies in all farms except one in Argentine. Meanwhile, some Ovelo Colorado breed were very near to Holstein but from some other farm it is most far from Holstein within the Bos Taurus breed. Moreover, Japanese Wagyu and Chilean Wagyu are resembled but Chilean Wagyu also had the characteristic of Angus. These frequency data provide infrastructure for understating the host factor of disease susceptibility.