GENETIC TOOLS FOR IDENTIFICATION OF FLAXES, CHIA, AND SESAME INGREDIENTS IN SEEDS AND PROCESSED FOODS

BRUNO, MARÍA CECILIA; WUNDERLIN, DANIEL ALBERTO; ZAPPA, MARÍA EUGENIA; PERAL GARCÍA, PILAR; POSIK, DIEGO MANUEL; GIOVAMBATTISTA, GUILLERMO

Nantes

Conferencia; 5th FoodIntegrity Conference Assuring the integrity of the food chain: Delivering real world solutions; 2019

Eurofins

Food integrity isan increasing concern around the world. Since the sanction and implementationof food laws to protect the consumers, several methods have been developed todetect and trace adulteration food, including DNA analysis. In the presentstudy, we used a High Resolution Melting real time Post PCR (HRM) analysis andEnd Point PCR using specie specific primers to evaluate genetic markers ascandidates for food authenticity in Chia (Salvia hispanica), Sesame (Sesameindicus) and Flax (Linum usitatissimum) species identification in foodcontaining seeds. Samples seeds of the three species were obtained from grocerystores in Argentina, Mexico and Italy. DNA extraction was a combination betweena mechanic lysis of seed and the DNA purification from the grinding material.HRM was performed with primers for the chloroplast ribulose-1,5-bisphosphate(RBCL). The HRM analysis can discriminate between the Chia, Flax and Sesamespecies individually and its presence in complex food matrix. However, themethod cannot be appropriated in matrix food containing a mixture in differentproportion of the three species in simultaneous. Whereas, EP PCR, is simple andcost effective alternative approach to the HRM analysis, especially for thosesamples that has mixtures of more than two different species.  Target NGS assays are carrying out in orderto contribute to the knowledge of different techniques in the agro foodintegrity.