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Individual radiosensitivity is an individual characteristic associated with an increased reaction to ionizing radiation. The purpose of our work is to establish a dose response curve useful to classify individuals as radiosensitive or radioresistant. Thus, a dose-response curve has been constructed by measuring the in vitro responses to increasing doses (0 to 8 Gy) of gamma radiation in the comet assay. The obtained curve fit well with both a lineal and polynomic equations in the range of 0 to 8 Gy. The overall dose-response curve has been constructed for the percentage of DNA in tail, as a measure of the genetic damage induced by irradiation. To probe the goodness of the constructed curve, a validation study was carried out with whole blood from two donors in a blind study. Results show that, for the two applied doses (2 and 6 Gy), the obtained values fit well inside the interval of confidence of the curve. As conclusion, our results prove the usefulness of the comet assay in determining individual responses to defined doses of gamma radiation. The standard dose-response curve constructed can be used to detect individuals departing from reference values.