IGEVET 21075
INSTITUTO DE GENETICA VETERINARIA "ING. FERNANDO NOEL DULOUT"
Unidad Ejecutora - UE
artículos
Título:
Influence of manganese on apoptosis and glutathione content of cumulus cells during in vitro maturation in bovine oocytes
Autor/es:
JUAN PATRICIO ANCHORDOQUY; JUAN MATEO ANCHORDOQUY; SEBASTIÁN J. PICCO; MATÍAS A. SIRINI; ANA LÍA ERRECALDE; CECILIA C. FURNUS
Revista:
CELL BIOLOGY INTERNATIONAL
Editorial:
ACADEMIC PRESS LTD-ELSEVIER SCIENCE LTD
Referencias:
Lugar: Aberdeen; Año: 2014 vol. 38 p. 246 - 253
ISSN:
1065-6995
Resumen:
We have investigated the effect of different Mn concentrations on (1) DNA integrity of cumulus cells by olive tail moment (OTM); (2) cumulus cells apoptosis by Annexin V staining assay; (3) intracellular total glutathione (GSH-GSSG) content; and (4) oocyte nuclear maturation and embryo cleavage after in vitro fertilisation (IVF). For this purpose, 0 (control), 2 (Mn1), 5 (Mn2) and 6 ng/mL (Mn3)Mn concentrations were added to IVM medium. Comet assay analysed by OTMwas significantly higher in cumulus cells arising from COCs matured without Mn (control, P
