IGEVET 21075
INSTITUTO DE GENETICA VETERINARIA "ING. FERNANDO NOEL DULOUT"
Unidad Ejecutora - UE
artículos
Título:
Expression of p24 gag protein of Bovine leukemia virus in insect cells and its use in immunodetection of the disease
Autor/es:
LARSEN A; GONZÁLEZ ESTER TERESA; SERENA M; ECHEVERRIA MG; MORTOLA E
Revista:
MOLECULAR BIOTECHNOLOGY
Editorial:
HUMANA PRESS INC
Referencias:
Lugar: Oregon; Año: 2013 vol. 54 p. 475 - 483
ISSN:
1073-6085
Resumen:
Abstract Bovine leukemia is a common retroviral infection of cattle. The disease is characterized by a strong immunological response to several viral proteins, but the antibodies against p24 and gp51 are predominant. In this study, a recombinant baculovirus containing the gag gene p24 was constructed and the protein, used as antigen, analyzed by western blot and an indirect in-house rp24- ELISA test. This allowed detecting the presence of antibodies for bovine leukemia virus in a panel of cattle sera. The authentication of the protein expands its potential use for different medical applications, from improved diagnosis of the disease to source of antigens to be included in a subunit vaccine
