CONICET | Buscador de Institutos y Recursos Humanos

Invertase is an enzyme that catalyzes the hydrolysis of substrates as sucrose to produce a 1:1 mixture of glucose and fructose sweeter than sucrose called invert sugar. It is mainly used in the food and pastry industry. Saccharomyces and Aspergillus, have developed to be good producers of this enzyme. The aim of this project is to optimize the culture parameters for the production of Invertase by Saccharomyces and Aspergillus and carry out enzymatic activity analyzes. The strains of Aspergillus niger (NRRL1419) and Saccharomyces cerevisiae (strain 2) were grown in Sabouraud liquid medium. The strains were suspended in acetate acetic buffer 0,1M/NaCl 0,5M pH 5.The effect of sonication on enzyme production was evaluated. Some samples were sonicated to break down cells and obtain greater enzyme production. Both strains were exposed to an induction process in sucrose solution 10 g/dm3 . Finally, the glucose oxidase method was used to measure the activity of the enzyme. Protein concentration was determined by spectrophotometry (505 nm). The strains of Saccharomyces cerevisiae and Aspergillus niger that were subjected to an induction process with sucrose solution showed higher activity values than those strains that were suspended in extraction buffer, obtaining values of 283 U/min for S. cerevisiae strain 2 and 4802 U/min for A. niger NRRL 1419 using sacarose solution. All enzyme activity values for samples without sonication were higher than those samples with sonication. Values calculated using extraction buffer were 44 U/min for samples with sonication and 113 U/min without sonication for strain 2 of S. cerevisiae. For A. niger NRRL 1419 the values were 46 U/min with sonication and 246 U/min without sonication.It is an easily reproducible system, and high enzyme activity values were obtained.There was no inhibition problems during the process.