Inhibition of naphthoquinones on biofilm formation of a foodborne pathogenic strain by oxidative stress

CELINA E. GARCÍA; NATALIA I. DI MARCO; CECILIA LUCERO ESTRADA; PAULINA PAEZ; CARLOS RODOLFO PUNGITORE

La Laguna, Tenerife

Congreso; COST Action CM1407; 2018

COST

Naphthoquinones are widely distributed in nature and they have been used inphytomedicine because their wide variety of pharmacological properties [1]. In previousstudies in our laboratory, we found that a group of new naphthoquinones, obtained by chemicalsemi-synthesis, inhibited biofilm formation of pork sausage and Yersinia enterocolitica strainwas isolated by more than 80%. It is known that bacteria have a balance between reactiveoxygen byproducts, named as reactive oxygen (ROS) and nitrogen species (RNS), andantioxidant defenses (AXO). When this balance is altered in favor of oxidants, oxidative stressis generated [2], which causes irreparable cellular damage. The aim of this work was evaluatethe relationship between the mechanism of action of 4 inhibitors naphthoquinones (Figure 1)of Yersinia enterocolitica bioserotype 1A / O: 7,8-8-8,19 (CLC001) biofilm formation withoxidative stress production. The generation of ROS was quantified by spectrofluorometry usingthe fluorescent probe H2-DCFDA (6-carboxy-2 ', 7'-dichlorodihydrofluorescein diacetatediacetoxymethyl ester) and the generation of RNS was quantified by Griess reaction. Todetermine AXO, the enzyme superoxide dismutase (SOD) activity was quantified bymethionine, riboflavin, EDTA and NBT assay.In this communication, the results of this study will be discussed.References:[1] Pinto A., de Castro S. (2009). Molecules 14: 4570-4590.[2] Dixon S.J., Stockwell B.R. (2014). Nat. Chem. Biol. 10: 9-17.