Stereoselective bioreduction of bulky-bulky ketones by the wild yeast Rhodotorula sp. LSL

AGUIRRE PRANZONI CB; BISOGNO FB; ORDEN AA; KURINA-SANZ MB

Buzios

Congreso; VII Workshop on Biocatalysis and Biotransformations and 1o Simposio Latinoamericano de Biocatalisis y Biotransformaciones; 2014

CatBiotrans

Although prochiral ketone bioreductions are widelyused to generate optically pure sec-alcohols,stereoselective reduction towards bulky aromaticsubstrates are still rare. Most of the selective ADHspossessing a broad substrate spectrum resemblessmall?bulky ketones, thus, the ?small? substituent islimited to a sterically non demanding group such asmethyl, ethyl, or chloromethyl [1]. Only a feworganisms or enzymes have been reported for thereduction of bulky-bulky ketones [2, 3]. Rhodotorulasp. LSL is a chemo extremophile wild microorganismisolated from a landfarming. We have alreadyshowed its ability for the stereoselective reduction ofa series of non-sterically demanding ketones, and itsuse as a lyophilized catalyst in one potchemoenzymatic procedures was optimized [4]. Sinceto generate of stereogenic centers at the endgameof the synthesis of pharmaceutically potentialproducts is an ongoing hot topic, we chose -ketotriazoles as substrates to identify the ability ofthis new catalyst for the reduction of bulky?bulkyketones.