Natural trypanocidal product produced by endophytic fungi through co-culturing

MAGALLANES-NOGUERA, CYNTHIA ALEJANDRA; CALDAS, IVO SANTANA; DO NASCIMENTO, JAINARA SANTOS; KURINA-SANZ, MARCELA; LUIZ, JAINE HONORATA HORTOLAN; SILVA, FELIPE MOURA; DOS SANTOS, ELDA GONÇALVES; SILVA, ELIANE DE OLIVEIRA

FOLIA MICROBIOLOGICA

SPRINGER

Lugar: Berlin; Año: 2019

0015-5632

Endophytic fungi live inside vegetal tissues without causing damage to the host plant and may provide lead compounds for drugdiscovery. The co-culture of two or more endophytic fungi can trigger silent gene clusters, which could lead to the isolation ofbioactive compounds. In this study, two endophytic strains isolated from Handroanthus impetiginosus leaves, identified asTalaromyces purpurogenus H4 and Phanerochaete sp. H2, were grown in mixed and axenic cultures. The meroterpenoid austinwas detected only in the extracts from the mixed culture. Once isolated, austin displayed very interesting trypanocidal activity,with an IC50 value of 36.6 ± 1.2 μg/mL against Trypanosoma cruzi in the epimastigote form. The results obtained highlight theimportance of the co-culturing of endophytic fungi to obtain natural bioactive products. The findings also enhance our understanding of the ecological relationships between endophytic fungi