Multiple response optimization of heat shock process for separation of bovine serum albumin from plasma

MENENDEZ, C.; GONZALEZ, U.A.; RIGAU, J.; SAITUA, H.A.

SEPARATION SCIENCE AND TECHNOLOGY

TAYLOR & FRANCIS INC

Lugar: Londres; Año: 2017 vol. 52 p. 1992 - 2001

0149-6395

Bovine serum albumin produced by heat shock from bovine plasma has been researched for the effects of sodium caprylate concentration [Cap], temperature (T), and pH on yield (Yield%) and purity (BSA%). Response surface methodology and desirability function approaches were applied to optimize its process. The best compromise solution was found with BSA% = 95.0 and Yield% = 28.5 for a [Cap] = 2 mM, T = 67.9°C, and pH = 5. A Monte Carlo simulation showed that it is possible to obtain excellent values for each individual response. However, a techno-economic feasibility study must be carried out to determine which one is the best option.