Proceeding: Insect growth regulatory effects of natural labdanes from Grindelia pulchella Dunal var. Pulchella

RETA, G.F.; GATICA, A.; SOSA, M.; DONADEL, O.J.; TONN, C.E.

BIOCELL

INST HISTOL EMBRIOL-CONICET

Año: 2011 p. 26 - 26

0327-9545

Growth Regulators (IGRs) can be powerful new tools of pest management. In previous studies we have reported the growth inhibitory bioactivity of grindelic acid isolated from plants. Taking into account the results of this compound as possible IGRs we evaluated the activity of labdanes against Tenebrio molitor L. (Coleoptera: Tenebrionidae) larvae as model insect. The objective of the present study was to test the activity of two compounds (grindelic acid and 1-hidroxy-grindelic acid) both isolated from G. pulchella. Aerial parts of the plant (3 kg.) collected in Dique Cruz de Piedra were extracted with ethanol and the extract obtained partitioned with a Si-Gel 60G column, using as eluent solutions of n-hexane and ethyl acetate in increasing polarities. 9.426 g of grindelic acid y 0.725 g of 1-hidroxy-grindelic acid were recovered. Labdanes isolated were assayed by topication on the fifth instar larvae (2μ/larvae dose and 10 μg/μl concentration). These compounds produced several deficiencies in development and mortality. The results suggest that these compounds could be considered as a potential alternative to control of stored product insects and they could be used in agricultural applications together with other approaches (Pest Management Integrated).