Circadian study of antioxidant defense system in the hippocampus of aged rats subjected to caloric restriction.

ALTAMIRANO FERNANDO; DELGADO MARCELA; FERRAMOLA MARIANA; LACOSTE GABRIELA; CASTRO PASCUAL IVANNA; ANZULOVICH ANA CECILIA

Cordoba

Congreso; XXXIII Annual Meeting and SAN; 2018

Oxidative stress plays a critical role in brain aging. Caloric restriction (CR) is the most accepted approach to slow the aging process and delay many age-related diseases. Previously, we showed circadian rhythms in the expression and activity of antioxidant enzymes in the hippocampus of young rats, that were abolished in the old animals. In the present work, we investigated temporal patterns of catalase (CAT) and glutathione peroxidase (GPx) expression and activity, as well as Nrf2 mRNA levels, in the hippocampus of aged rats under CR. Holtzman male rats were fed with a diet reduced by 40% in calories during the last 3 months prior to the 22 months of age. The mRNA levels were determined by RT-PCR and the enzymatic activity were evaluated by kinetics assays in hippocampi isolated every 4 h during a 24h-period. Interestingly, we observed CR restored the circadian rhythmicity of all the studied parameters (Chronosfit: p