Circadian study of Dnmt1, BDNF and TrkB expression in the cerebellum of rats subjected to caloric restriction

FERRAMOLA, M.; ANZULOVICH AC; CASTRO-PASCUAL, I; LACOSTE MG; ALTAMIRANO FG; DELGADO SM

Valparaíso

Simposio; XIV Latin American Symposium on Chronobiology 2017 - LASC 2017; 2017

We aimed to evaluate the circadian variation of DNA methyltransferase 1 (Dnmt 1), Bdnf and TrkB expression, in the cerebellum of aged rats subjected to caloric restriction. Holtzman 3- and 22-month old male rats and 22-month old male rats under caloric restiction were maintained in constant darkness conditions during 15 days, in order to study the effects of caloric restriction on endogenous rhythms. Previously, we observed, that TrkB and Dnmt1 mRNA levels varied throughout a 24h period (p