ANDROGEN DEPRIVATION AND LUNG DISEASE RISK

BOLDRINI GG; BIAGGIO VS; PEREZ CHACA MV; PIGUILLEM SN; ALVAREZ SM; MARTIN MOLINERO GD; CIMINARI ME; GOMEZ NN

Mendoza

Congreso; Reunión Anual de la Sociedad De Biología de Cuyo; 2016

Sociedad de Biología de Cuyo

Previously, we demonstrated that inflammatory parameters changed in lung parenchyma after castration. The antioxidant response elements (ARE) are involved in gene activation coding for a number of antioxidant proteins. Androgen deprivation triggers of oxidative stress and exacerbates lung injury. We studied if castration produced changes in oxidative markers and inflammation parameters.Male Wistar rats (200 ± 20 g) were separated in 3 lots: controls (Co), castrated (Ca), and castrated supplemented with testosterone (Ca+T) for five days. 60 days after castration rats were killed and lungs were obtained. Before that, bronchoalveolar lavages (BAL) were collected and cell count was performed. RNA was extracted by using TRIzol. mRNA levels of TGF-β, HO-1, Neuregulin (NRG-1), VCAM, COX2 and FOXO3a were quantified by RT-PCR. ANOVA was used for statistical analysis. After 60 days of castration, COX2 significantly increased its expression in Ca group (p