CADMIUM ALTERS APOPTOSIS MARKERS AND MORPHOLOGY IN RAT LUNG. EFFECTS OF DIFFERENT PROTEIN SOURCES

BOLDRINI GABRIEL GIEZI; ALVAREZ SILVINA M; PLATEO PIGNATARI MARIA G; MARTIN MOLINERO GLENDA D; GIMENEZ MS.

TUCUMAN

Congreso; Reunión Conjunta de Sociedades de Biología de Argentina; 2015

Cadmium (Cd) is a toxic metal and an important environmental contaminant. We studied its effects on apoptosis markers and the histoarchitecture of rat lung under different diets. 4 lots of female Wistar rats were used: 2 lots received casein (Cas) and 2 lots soybean (Soy) as protein sources. Within each group, 1 lot received regular water (control-Co) and the other, 15 ppm of Cd in the drinking water for 60 days. Total RNA was isolated with Trizol and cDNA was obtained. Transforming growth factor beta (TGF-β), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), p53, BAX and Bcl-2 were determined by PCR. S28 was used as the control. The lungs were fixed, sectioned, stained, and examined for evidence of lung injury. TGF-β expression decreased in Soy-Co and Soy-Cd groups vs their respective Caseine groups (p