Impact of Western Diet-Induced Obesity on Mouse Lung Inflammation and Oxidative Stress

MARIA C DELLA VEDOVA , MARTIN RINALDI TOSI1, MARCOS D MUÑOZ1,3, SILVINA GARCIA1, SANDRA E GOMEZ MEJIBA1,3, AND DARIO C RAMINEZ1,2

Seattle

Congreso; SFRBM`s; 2014

Sociedad de Radicales Libres

Impact of Western Diet-Induced Obesity on Mouse Lung Inflammation and Oxidative Stress Maria C Della-Vedova1,2, Martin Rinaldi Tosi1, Marcos D Muñoz1,3, Silvina Garcia1, Sandra E Gomez Mejiba1,3, and Dario C Raminez1,2 1Lab. Exp. Med & Ther., Imibio-Sl, Natl. Bureau of Sci & Tech. Res. (CONICET), Argentina, 2Department of Biochemistry, School of Chemistry, Biochemistry & Pharmacy, Natl. University of San Luis, Argentina, 3Department of Nutrition, School of Health Sciences, Natl. University of San Luis, Argentina Obesity is a chronic inflammatory disease caused by a positive energy imbalance that may negatively impact the lung. Previously, we have characterized an obesity model in male B6 mice by feeding them for 16 weeks a diet rich in chicken fat and supplemented with 10% fructose in the drinking water (HFD+F) and compared it with other diets (i.e., high-fat diet only (HFD); low fat diet (LFD) and LFD plus fructose (LFD+F)). Herein we compared the effects of different diets on the redox and inflammatory status in the mouse lungs. We hypothesize that adipogenic effects of a HFD+F diet may cause oxidative stress and inflammation in the lung. To test our hypothesis, after the diet period animals were euthanized and lung tissue was removed and analyzed. Our data (mean􀁲SEM) show that a HFD+F diet: i) reduced antioxidant capacity (AC, uM L-ascorbate, HFD+F:2106±166 vs HFD: 2574±169; LFD+F:2756±205; LFD: 3416±191); ii) increased catalase activity (IU/mg/proteins, HFD+F:320±32 vs HFD:230±33; LFD+F:215±31; LFD:160±32); iii) reduced GSH/GSSG molar ratio (HFD+F0.008􀁲0.001 vs HFD:0.010􀁲0.001; LFD+F:0.013􀁲0.005; LFD:0.015􀁲0.003; iv) increased protein carbonyls (nmol/mg proteins, HFD+F:35.75±1.53 vs HFD:31.13±2.69; LFD+F:29.58±1.01; LFD:24.35±2.78); v) increased TBARS content (umol MDA/mg proteins, HFD+F:3.30±0.29 vs HFD:1.63±0.15; LFD+F:0.75±0.09; LFD:0.45±0.06); vi) increased nitrites (nmol/mg proteins, HFD+F:14.79±2.39 vs HFD:10.14±1.41; LFD+F:8.20±1.07; LFD:4.87􀁲1.13); vii) increased TNF-alfa (pg/mg proteins, HFD+F:62.21±8.70 vs HFD:43.88±1.92; LFD+F:31.96􀁲2.67; LFD:22.72±3.57) and viii) increased MPO activity (IU/mg tissue, HFD+F:0.020±0.003 vs HFD:0.012± 0.002; LFD+F:0.010±0.002; LFD:0.006±0.001). Our data suggest that western diet-induced obesity causes oxidative stress and inflammation in the lung with increased the retention of neutrophils. Supported by PROICO-2-3214(FQBYF)/PROICO10-0414(FCS)- UNSL