IS THE TIME VARIABLE IMPORTANT IN THE EFFECT OF ANDROGEN DEPRIVATION IN LUNG?

ESCOBAR CORREAS SOPHIE MELANIE; PACHECO GUIÑAZU ANAHI BELEN; ALVAREZ SILVINA MONICA; BIAGGIO VERONICA SILVINA; CIMINARI MARIA EUGENIA; PEREZ CHACA MARIA VERONICA; PIGUILLEM SILVANA; GOMEZ NIDIA NOEMI

Mendoza

Congreso; Reunión Anual de la Sociedad de Biología de Cuyo; 2013

IS THE TIME VARIABLE IMPORTANT IN THE EFFECT OF ANDROGEN DEPRIVATION IN LUNG? Escobar Correas SM, Pacheco Guiñazú AB, Álvarez SM, Biaggio VS, Ciminari ME, Pérez Chaca MV, Piguillem SN, Gómez NN. Lab de Anatomía y Fisiología, UNSL, IMIBIO-CONICET, San Luis. Argentina. ngomez@unsl.edu.ar Lungs are susceptible to injury due to free radicals. Also, androgens play a physiological role in pulmonary function. We tested the effect of castration on the prooxidant-antioxidant balance at two different times: 30 and 60 days after castration. Wistar male rats (200±20g) were separated in three groups: controls (Co), castrated (Ca), and castrated supplemented with testosterone (Ca+T) for five days. After 30 (30d) or 60 days (60d) rats were killed. TBAR?S and CAT activity were determined. Total RNA was isolated and Androgen Receptor (AR), Superoxide Dismutase (SOD), Nuclear factor-erythroid 2 related factor 2 (nrf-2), Glutathion peroxidase (GPx) and NADPH Oxidase (NOX-2) were determined by PCR. S28 was the control. The expression of AR showed an increase in Ca and a significant decrease in Ca+T in both time groups (p