IMIBIO-SL   20937
INSTITUTO MULTIDISCIPLINARIO DE INVESTIGACIONES BIOLOGICAS DE SAN LUIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Paracellular absorption of nutrients in bats is high during intestinal luminal perfusions
Autor/es:
PRICE, E. R.; BRUN, A; FASULO, V.; KARASOV, W. H; CAVIEDES-VIDAL, E.
Lugar:
San Francisco
Reunión:
Congreso; SICB Annual Meeting; 2013
Resumen:
Water-soluble nutrients can be absorbed across enterocytes via
protein-mediated transport, or paracellularly through the tight
junctions between enterocytes. Previous in vivo measurements
of bats that were orally dosed with carbohydrate probes have
shown that bats absorb larger proportions of nutrients
paracellularly than similarly-sized non-flying mammals. While
this could indicate greater paracellular permeability of the
intestinal epithelium, it could also be caused by longer
retention time or slow gastric evacuation. We sought to
determine if bat intestines are particularly permeable to
nutrient-sized molecules. We performed in situ intestinal
luminal perfusions on Tadarida brasiliensis and Myotis
lucifugus. We cannulated the intestine and recirculated an
isosmotic buffer containing 10-75 mM D-glucose, 10-75 mM
proline, and two carbohydrate probes that are only absorbed
paracellularly, 1 mM L-arabinose, and 1 mM lactulose, and
radioisotope tracers for these molecules. Absorption of
arabinose (MW 150) was nearly double that of lactulose (MW
342), demonstrating a similar molecular size sieving effect as
has been seen previously for various species in vivo. At low
molarity proline conditions, paracellular absorption (assessed
by arabinose clearance) can account for at least 44% of total
proline absorption. At 75 mM proline, paracellular absorption
accounts for a majority of proline absorption. These data
demonstrate that insectivorous bats rely heavily on paracellular
absorption for the uptake of nutrients and confirms the high
intestinal permeability suggested by whole-animal studies.
Supported by NSF Award 1025886.