Rat ovary exposed to Cadmium – Effect of soybeans as protein source

ÁLVAREZ SM, CALDERONI AM, PLATEO PIGNATARI G AND GIMÉNEZ MS.

Congreso; Sociedad de Biologia de Cuyo; 2012

Rat ovary exposed to Cadmium – Effect of soybeans as protein source Álvarez SM, Calderoni AM, Plateo Pignatari G and Giménez MS. Area de Quimica Biologica. Univ Nac San Luis. 5700 - San Luis. Argentina. salvarez@unsl.edu.ar Cadmium (Cd) is a toxic element considered as an important environmental contaminant, and a major constituent of tobacco smoke. The aim was to study the effect of chronic exposure to cadmium on the lipidic profile of rat ovaries and the incidence of different diets: one casein based and one soybean based. 4 lots of afult female Wistar rats were formed: 2 lots received casein and 2 lots soybeans as protein source in the diet. Within each protein group, 1 lot received water without Cd (control) and the other received 15 ppm of Cd (as Cl2Cd) in the drinking water, ad libitum for 60 days. Lipid extraction from ovaries was made with isopropanol: hexane 3:2. Lipids were separated by TLC using hexane:ethylic ether:acetic acid 80:19:1. We measured total cholesterol (TC), triglycerides (TG) and phospholipid contents (PL). Proteins were measured by Lowry. The results are media ± SEM. TC (g/mg protein): Casein Control: 5.9790.44; Casein Cd: 18.361.62; Soy Control: 21.18±3.17 and Soy Cd: 14.79±0.07 (p