Cadmium exposition on antioxidant enzyme activities in aorta. Effect of soy bean as protein source in the diet.

PÉREZ DIAZ MF, FERRAMOLA ML, OLIVEROS L, GIMÉNEZ MS.

Mendoza

Congreso; SOBIO 2010; 2010

Sociedad Argentina de Biologia de Cuyo

Cadmium exposition on antioxidant enzyme activities in aorta. Effect of soy bean as protein source in the diet. Pérez Diaz MF, Ferramola ML, Oliveros L, Giménez MS. Dpto. Bqca. y Cs. Biológicas,UNSL. IMIBIO- CONICET- UNSL. Chacabuco917. 5700-San Luis. E-mail:matiaspdiaz@hotmail.com   Cadmium (Cd) is an environmental pollutants that causes lesions in different organs, depending of exposure dose, time and administration route. We have showed that Cd modifies the aorta lipid contents. Now, we investigated the effect of administration in the drinking water of 15 and 100 ppm of Cd, as CdCl2, for 2 months, on lipoperoxidation and antioxidant enzyme activities in aorta of adult male Wistar rats. Animals were maintained on isocaloric diets containing casein (Ca) or soy bean (So), as protein source. Rats without Cd treatment were used as controls (Ca and So groups). Spectrophotometric assays were used to measure TBARs levels (as MDA), and superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) activities, in aorta. Also, TBARs levels and paraoxonase (PON-1) aryl-sulphatase activity were determined in serum. On Ca diet: Cd  100 ppm, but not Cd 15 ppm, increased SOD and CAT activities in aorta, and TBARs levels (p<0,05) and PON-1 activity in serum, compared with Ca group. On So diet: Cd 15 ppm, increased GPx  activity and TBARs in aorta, and Cd 15 and 100 ppm decreased PON-1 activity in serum (p<0,0001), compared with So group. All the So groups showed higher TBARs content in aorta than their respective Ca groups. Soy bean in the diet was not able to protect the aorta against redox changes induced by Cd.