Circadian rhythms of luteal regression-involved factors are modified in TNFRp55-deficient mice

DELSOUC, MARÍA B.; VALLCANERAS, SANDRA; DE LA VEGA, MAGALÍ; RAGUSA, VICENTE; CASAIS, MARILINA; PONCE IVANA; ANZULOVICH, ANA C.

REPRODUCTION FERTILITY AND DEVELOPMENT

CSIRO PUBLISHING

Lugar: Collingwood; Año: 2018 vol. 30 p. 1651 - 1665

1031-3613

A proper rhythm of Luteal regression-involved factors is crucial for determining the physiological duration of the estrous cycle. The p55 Tumor Necrosis Factor receptor (TNFRp55) mediates most of the TNFα effects and taking into account the role of TNFα in luteal function and circadian regulation, our objectives were to analyze in the ovary of mice in the luteal regression phase: whether progesterone (P4) levels and its metabolizing enzymes expression are circadian and endogenous; to investigate the circadian oscillation of clock genes expression; to investigate whether key gene expression of apoptosis and anti-oxidant mechanisms vary daily; and finally to evaluate the consequences of TNFRp55 deficiency. We found P4 oscillates circadianally following endogenous rhythms of clock factors. Noteworthy, TNFRp55 deficiency modifies circadian rhythms of P4 levels and its metabolizing enzymes, probably, as a consequence of changes in the circadian oscillation of Bmal1 and Cry1. The absence of TNFRp55 modified the circadian rhythms of apoptosis genes and also affected the circadian parameters of anti-oxidant enzymes and levels of peroxidation in the ovary in diestrous. This work will strengthen the hypothesis that a deregulation of TNFα signaling may be a potential cause for altered circadian and menstrual cycling in some gynecological diseases.(Impact Factor: 2.105)