IFEC   20925
INSTITUTO DE FARMACOLOGIA EXPERIMENTAL DE CORDOBA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
ACTIVATION OF CANNABINOID CB1 RECEPTOR WITHIN NUCLEUS ACCUMBENS CORE UNDERLIES RESTRAINT STRESSINDUCED REINSTATEMENT IN EXTINGUISHED COCAINE?CONDITIONED ANIMALS
Autor/es:
GUZMAN, A.S.; DE GIOVANNI, L.N.; VIRGOLINI, M.B. ; CANCELA, L.M.
Lugar:
Huerta Grande, Córdoba, Argentina.
Reunión:
Congreso; XXIX Congreso de la Sociedad Argentina de Investigación en Neurociencias.; 2014
Institución organizadora:
Sociedad Argentina de Investigación en Neurociencias
Resumen:
Endocannabinoid system is implicated in relapse in extinguished cocaine-conditioned animals. Moreover, Nucleus Accumbens (NAc), a high density cannabinoid CB1 receptors brain region, is involved in cocaine and stress-induced reinstatement. Previous results from our lab demonstrated that in extinguished cocaine-conditioned animals evaluated in a conditioned place preference test (CPP), the restraint-stress was able to reinstate the cocaine CPP and that phenomenon depends on the glutamatergic transmission within NAc. Additionally, in our lab it has been revealed that intra-Core administration of AM251, a CB1 antagonist, abrogated that restraint stress-induced reinstatement. According to the previous protocol, on the reinstatement test day, extinguished cocaine-conditioned Wistar rats were microinjected intra-Core with a CB1 agonist, ACEA, (0, 0.001 or 0.01 fmol/side) or vehicle, and subsequently assigned to the following treatments: 1) Stressed animals: 15 min-restraint exposure, a non-reinstatement session, and 2) Control animals. The administration of ACEA facilitated the restraint stress-induced reinstatement in extinguished cocaine-conditioned animals that were exposed to 15 min of stress. Our results support the hypothesis of the influence of CB1 receptors in restraint stress-induced reinstatement. Future studies will focus on a possible glutamate dependent mechanism within NAc Core to explain the effect of CB1 receptor activation on restraint stress-induced reinstatement