GPR162 is expressed in the hypothalamus and is involved in food intake related behaviour and glucose homeostasis

SMITHA SREEDHARAN; VALERIA P. CARLINI; JOSEFIN A. JACOBSSON; PAWEL K.; OLSZEWSKI; TATJANA HAITINA; JOHANNA HAMMER; OLGA STEPHANSSON; PHILIP YSTRÖM; WOLFGANG SOMMER; ULF RISERUS; ALLEN S. LEVINE; LARS LANNFELT; CLAUDE MARCUS; MARKUS HEILIG; SUSANA R DE BARIOGLIO; ROBERT FREDRIKSSON; HELGI B. SCHIÖTH1

FEBS JOURNAL

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2012 vol. 278 p. 4881 - 4894

1742-464X

The Rhodopsin family of G protein-coupled receptors (GPCRs) includes about 270 non olfactory receptors and is the largest family of GPCRs. About sixty non olfactory Rhodopsin GPCRs are still orphans without known ligands and fairly little is known about their functions. In this study, we present molecular, neuroanatomical, genetic and behavioral data implicating Rhodopsin family protein GPR162 in regulation involved in food intake related behaviour and glucose homeostasis. The real-time PCR data shows that GPR162 is predominantly expressed in CNS and negligible in peripheral tissues. The in situ results confirmed significant expression of GPR162 in several hypothalamic sites, amygdala, substantia nigra and ventral tegmental area among others regions. In line with the distribution of the GPR162 mRNA in feeding circuitry, antisense oligo knockdown of GPR162 showed a significant reduction in food intake and a strong trend toward a reduction in body weight in rats. The human genetics suggests that genetic variants of GPR162 affect glucose homeostasis. In conclusion, this study provides evidence linking the orphan GPR162 gene with the regulation involved in food intake related behaviour and glucose homeostasis.