Aberrant glycosylation during Inflammatory Bowel Diseases: mechanistic insights into the role of miRNAs and hypoxia

GAMBARTE J; CROCI DO; BANNOUD N; MARIÑO KV; GARCIA PA; RABINOVICH GA

CABA

Congreso; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología (SAI); 2020

SAIC/SAI/SAFIS

Even though alteration of the intestinal glycome during IBD has been known for many years, factors modulating and triggering thesechanges are still unclear. Recently, heightened hypoxia has been described as an important environmental factor in IBD, and microRNAs (miRNAs) have emerged as critical mediators of gene expression; however, a connection between them in IBD has not been thoroughly explored. Our objective is to unveil molecular and environmental factors modulating the intestinal glycome during IBD. We started by analyzing data from several miRNAs databases, searching for those dysregulated in IBD, hypoxic conditions and that could modulate inflammation, angiogenesis and glycosylation. Cross data analysis showed candidate miRNAs mir-155, mir-146 and mir-124 as regulators of several glycosyltransferases (ST6GAL1, ST6GAL2, FUT8, FUT11, MGAT4, MGAT5) and the mannosidase MAN1A1. By using publicly available datasets from IBD patients (E-MTAB-2967) we found that ST6GAL1, STGAL2, FUT8 y FUT11 were upregulated (p