IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Study of oxidative stress and antioxidant defense against acude mercury exposure in the bioindicator gasteropod Pomacea canaliculata
Autor/es:
CAMPOY DÏAZ, ALEJANDRA D;; MALANGA, GABRIELA; VEGA, ISRAEL A
Lugar:
Lima
Reunión:
Congreso; XI Congreso Latinoamericano de Malacología; 2020
Institución organizadora:
Asociaciones Malacológicas de Latinoamérica
Resumen:
The apple snail Pomacea canaliculata has been proposed as a sentinel species of mercurypollution in freshwater bodies. Although kidney of apple snails accumulated preferentially thiselement, the putative mechanisms involved in the detoxification of this oxidative element havenot studied yet. Here, we report the oxidative stress and oxidative defense in the snail?s kidneyafter exposure at a relevant ecologically Hg concentration. Adult snails were exposed 96 h to5.5 micrograms of Hg by liter of water. Snails were sacrificed at 0, 24, 48, 72 and 96 h afterexposure. Elemental concentration was analyzed by neutron activation. The oxidative stressand cellular damage were evaluated by oxidation rate of 2?7?-dichlorodihidrofluoresceinDiacetate (DCFH-DA), generation of lipid radicals (LR), and carbonyl groups concentration.Non-enzymatic (β- carotene; β-C) and enzymatic (glutathione-S-transferase, GST; catalase,CAT) antioxidant defenses were also evaluated. Hg concentration increased significantly(almost three times, compared with non-exposed) after exposure (96 h), which wasaccompanied by an increase in the LR generation (+157%) and DCFH-DA oxidative rate(+310%). Protein carboxylation (+160%) increased throughout exposure. The β-C content andthe CAT enzymatic activity remained approximately constant. The GST activity decreasedbetween 48 and 72 h, but returned to basal levels towards 96 h. Together, these findingsindicate that the Hg acute exposure generates an oxidative stress in the kidney of P.canaliculata, overcoming the antioxidant defenses of the animal and causing damage to lipidsand cellular proteins.