The Effect of Dehydroleucodine in Adipocyte Differentiation

A. GALVIS; A. MARCANO; H. PRIESTA; L.A. LOPEZ; A. BARBIERI

Philadelphia, PA. USA

Congreso; 50 th Annual Meeting The American Society for Cell Biology; 2010

American Society for Cell Biology

Dehydroleucodine (DhL), a sesquiterpene lactone of the guaianolide group, was isolated from aerial parts of Artemisia Douglasiana Besser. Recent studies have demonstrated that DhL inhibited the proliferation of vascular smooth muscle cells.  In this study we examine the effect of DhL in adipocyte differentiation. To investigate the effect of DhL in cell differentiation, 3T3-L1 preadipocytes were exposed to different concentrations of DhL (from 0.65uM to 10uM) during the differentiation process.  The addition of DhL inhibited significantly the lipid droplet accumulation in a dose-dependent manner and attenuated dramatically the protein of adipogenic transcriptional factors, C/EBPalpha and PPARgamma, during adipogenesis. Furthermore, the increase of phosphorylation and expression of ERk1/2 and Akt during adipocytic differentiation were not inhibited by treatment with DhL. Taken together, this data suggests an important inhibitory effect of dehydroleucodine in cellular pathways involved in adipocyte differentiation through the modulation of C/EBPalpha and PPARgamma pathway that play critical roles during adipogenesis.