IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Neurosteroids Regulate Indispensable Events For Fertilization In Human Sperm
Autor/es:
MATA MARTINEZ ESPERANZA; POBLETE SANTIAGO; JUSTRIBÓ GISELLA; DE BLAS, GERARDO ANDRÉS; ARIAS RODOLFO
Lugar:
San Luis
Reunión:
Congreso; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2019
Institución organizadora:
Sociedad de Biología de Cuyo
Resumen:
Fertilization is an essential event for the generation of a new individual and propagation of species. However, the functional changes that take place in the sperm during their trip to find and merge with the egg are not fully known. The sperm must undergo a long preparation process that occurs during their journey through the female genital tract. Three indispensable events must occur for fertilization: hyperactivation, capacitation and acrosome reaction (AR), which are regulated through a synchronized and precise communication between egg and sperm. Progesterone, the main steroid secreted by cumulus oophorus cells, exerts its effect through the activation of the specific calcium ion channel of the sperm: CatSper. The activity of this ionic channel is essential for the three essential events for fertilization to be carried out succesfully. Steroid hormones control very important functions in organisms such as development, metabolism, inflammation, ionic homeostasis and reproduction. Modulation of ion channels by steroid hormones has been reported in heart, neurons, smooth muscle and beta cells of the pancreas. On the other hand, it is known that progesterone, pregnenolone (PregNe), pregnenolone sulfate (PregN-S), allopregnanolone (AllopregNa) and cortisol are a set of neuroesteriodes that act as neuromodulators, articulating the cellular mechanisms of the central nervous system to regulate functions related to reproductive, cognitive and memory, are also associated with mood, behavior, anxiety and stress. Due to the function of these neurosteroids has not been evaluated in sperm physiology. In this work we set out to study the effect of these neurosteroids on motility, AR and intracellular Ca2+ mobilization in human spermatozoa. Our results indicate that PregNe, PregN-S and AllopregNa produced similar effects to progesterone in increasing intracellular Ca2 +, AR and modified motility parameters related to hyperactivation. For its part, cortisol neither induced an increase in intracellular Ca2 + nor AR, however it inhibited the progesterone-induced AR.