Dehydroleucodine inhibits migration of HeLa cells

AMAYA M.C; COSTANTINO V; BERTOLDI V; LOSSINO A; LOPEZ L.A

San Miguel de Tucumán, Argentina

Congreso; XLV Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB).; 2009

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

It is important to identify chemical compounds with cell antimigratory activity to control tumor metastasis. The present study was designed to test the effect of a sesquiterpene lactone, dehydroleucodine (DhL), on the migration of HeLa cells in culture. Before use, DhL solution was freshly prepared by diluting with DMEM F12 medium (medium) a stock solution of 0.2 M DhL in DMSO. For wound healing assay, cells were grown to confluent monolayer in medium supplemented with 10% fetal bovine serum (FBS) in a 12-well plate. After 24 h of incubation in serum free medium, the wound was made by scraping the cell monolayer with a pipette tip. The cells were cultured with 0-20μM DhL in medium supplemented with 1.5% FBS containing 2 mM hydroxyurea. Wounds were imaged at 0 and 24 h of treatment, at the same spots by direct microscopic visualization. Migration of cells in 0μM DhL was considered 100%. Cells treated with 5, 10 and 20 μM DhL migrated 93% ± 0.7, 75% ± 2.5 and 60% ± 5.5 (x ± SEM of 3 experiments) respectively. Recently, it has been shown in HeLa cells in culture, that DhL inhibits cell-cycle progression for arresting cells in G2. In this study, DhL effectively inhibited migration of HeLa in culture. DhL looks like a new promising candidate as an antimetastatic agent.