IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
EXPLORING AND IDENTIFYING ETIOLOGICAL AGENTS RESPONSIBLE FOR FOODBORNE DISEASES. Microbiological Quality of Minimally Processed Fruits and Vegetables in Great Mendoza?s Points of Sale
Autor/es:
CUERVO, MARÍA PAULA; DI SANTO, VANESSA; MICHAUT, MARCELA A.; TORTI, JERÓNIMO FACUNDO; OROZCO, A; PIZARRO, MARCELA A.; NARDELLO, ANDREA LIV; RABINO, DANIEL; MONTBRUN, MARIA DEL CARMEN
Lugar:
Mendoza
Reunión:
Simposio; II Simposio Internacional de Medicina Traslacional de la Facultad de Ciencias Médicas; 2019
Institución organizadora:
Facultad de Ciencias Médicas, Universidad Nacional de Cuyo
Resumen:
Introductionand objectives: Productionof ready-to-eat foods is a global trend, which has promoted the need toinvestigate their safety. Minimally processed fruits and vegetables are part ofthis growing social demand.This work objective was to obtain evidence of the microbiological quality ofminimally processed fruits and vegetables destined for direct consumption inthe cities of Mendoza, Guaymallén, Las Heras, Godoy Cruz and Luján de Cuyo.Methods, Results and Conclusions: Thepresence of E. coli producing Shigatoxin (STEC) and Salmonella sp. wasinvestigated in100 samples obtained between September 2016 and August 2018. Recountingof viable mesophilic microorganisms as product alteration indicators, totalcoliforms as hygiene indicators, and E.coli as faecal contamination indicators were carried out.Aerobic andfacultative mesophilic bacteria recounts were made at 30°C according to theInternational Standard Organization ISO 4833-2:2013 ANMAT 2014 procedure.Presence of Salmonella sp. was determined accordingto International Standard ISO 6579:2002, the presence of E. coli was investigated in L-EMB agar and the identity of thebacteria was confirmed by the IMViC tests.The presence ofShiga toxin-producing E. coli (STEC) was determined according to the Manual ofProcedures "Detection of STEC O157:H7 and non-O157 in food byimmunomagnetic separation and PCR", of the Physiopathogeny Service of theMalbrán Institute.Three samples were positive for STEC, one for Salmonella sp.Though the number of mesophiles is not regulated in Argentina`s Food Code (CAA)for this type of food, other countries suggest must be under 5.105CFU/g. 88% of the samples studied surpass these values.Therefore, thisresults shows the need to strengthen the epidemiological inspection andsurveillance system to ensure these products safety.