IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
ULTRASTRUCTURAL CHANGES IN THE ACCESOTY SEX GLANDS OF HYPERCOLESTEROLAEMIC MALE RABBITS. RECOVERY BY OLIVE OIL ADDITION TO DIET.
Autor/es:
SAEZ LANCELLOTTI, T.E.; COLOMBO REGINA; FORNES MW.; MONCLUS MA
Lugar:
Buenos aires
Reunión:
Congreso; Reunion SAMIC 2019 - CIASEM; 2019
Resumen:
ULTRASTRUCTURAL CHANGES IN THE ACCESOTY SEX GLANDS OF HYPERCOLESTEROLAEMIC MALE RABBITS. RECOVERY BY OLIVE OIL ADDITION TO DIET.COLOMBO R., MONCLUS M., SAEZ T., FORNÉS M.ANDROLOGICAL RESEARCH LABORATORY OF MENDOZA, INSTITUTE OF HISTOLOGY AND EMBRYOLOGY OF MENDOZA (LIAM - IHEM - CONICET MENDOZA), ARGENTINA. e-mail: mfornes@fcm.uncu.edu.arSemen of rabbits with hypercholesterolemia (HC), due to fat diet (DG), presents volume decrease, biochemical changes without pH changes. In turn, the spermatozoa express morphological and functional alterations. These abnormalities were corrected with the addition of olive oil (OO) to diet (1, 2, 3 and 4). The normal functioning of the attached glands, mainly prostate and seminal vesicle give the composition of the semen. It would be expected that changes in the seminal fluid are due to alterations of the male glands. To study the probable changes, prostate and seminal vesicles were processed from normal rabbits fed for more than 3 months with DG (14% fat) and / or with added fat (7%) and extravirgin OO (7%). The tissue samples were processed by usual technique for EM, using the fixative described by Mollenhauer et al. (1972), embedded in Eponate and observed in an EM Zeiss 902. The normal prostate was composed of a high epithelium of cylindrical cells and typical distribution of organelles of secretory cells, similar to seminal vesicle. While under fat diet the appearance of electron-dense vacuoles in the apical zone (v, lipids?) (Figs 1 and 2), mitochondria in fission or fusion (Fig. 1, m), "channels" between cells with microvesicular content (Fig. 2, c) were observed. Mixed bodies of variable density were also detected, neighbors of the RER (Fig. 3, box). These variations disappeared when incorporating OO. In the seminal vesicle the changes included more electron-lucid vacuoles, which also disappeared with the addition of OO. It is likely that these morphological changes affect the normal glandular function and thus contribute to the seminal alterations already described. Recovery of normal morphology by OO could also explain the semen improvement by OO.