ULTRASTRUCTURAL STUDY OF SEMINIFEROUS EPITHELIUM IN LIOLAEMUS CUYANUS THROUGHOUT REPRODUCTIVE ANNUAL CYCLE

BARAUMA A; QUIROGA, LORENA; DELLE COSTE M; SANABRIA, EDUARDO; ROMERO C; FORNES MW.

Buenos aires

Congreso; Reunion SAMIC 2019 - CIASEM; 2019

SAMIC - CISEM

ULTRASTRUCTURAL STUDY OF SEMINIFEROUS EPITHELIUM IN LIOLAEMUS CUYANUS THROUGHOUT REPRODUCTIVE ANNUAL CYCLEBARAUNA AA1, 6, DELLE COSTE M2, ROMERO C2, QUIROGA L3, 4, SANABRIA E3, 4, 5 y FORNÉS M1, 6 1Lab. de Inv. Andrológicas, Instituto de Histología y Embriología de Mendoza (LIAM ? IHEM ? CONICET Mendoza), Argentina. 2Universidad Juan Agustín Maza 3 Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET) 4Facultad de Filosofía, Humanidades y Arte, Universidad Nacional de San Juan, Argentina. 5Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Cuyo, Mendoza, Argentina. 6Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, Argentina. E-mail de contacto: andrea.barauna88@gmail.com In arid zones availability of resources is limited in space and time due to the marked climatic seasonality. As a reproductive strategy, vertebrates adapt through an endogenous circannual rhythm, comprised of a single reproductive period, followed by a stage of complete sexual inactivity. In adult males, there are changes in the ultrastructure of seminiferous epithelium (SE) implies different gonadal status. The goal of this work is to determine if the SE of the testicle in Liolaemus cuyanus, from the Mount Desert, San Juan Province, exhibits the predictive annual variation. During its reproductive season, 29 adult males were collected. One gonad of each specimen was isolated and fixed in 2% glutaraldehyde in PAF-buffer phosphate. The samples were processed by standard techniques for EM and were observed with a Zeiss 900 Microscope. The histological samples were analyzed with ImageJ free software, version 1.37v showing morphological measurements changes such as perimeter / surface of SE. Micrograph, presented in October, basal spermatogonia, and few spermatocytes in meiotic division, round spermatids with condensed nuclei and developing acrosomes (Fig. 1). In November, some spermatocytes developed in group and others continued the meiosis. By December, a great number of sperm were espermiated into the tubular lumen (Fig. 2). In January the differentiation was maintained but in February and particularly March, intraepithelial spaces, apoptosis stages and some electron-dense deposits began to be evident. The process of spermiogenesis shows particularities of the species, such as cluster of membranes-proteins associated with the flagellum. We can infer that L. cuyanus presents an annual cycle with active SE in spring-summer and a total regression prior to the beginning of autumn and winter, seasons in which the period of lethargy occurs.