IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
THE Ca2+ WAVE INDUCED BY 8-pCPT-2'-O-Me-cAMP IN HUMAN SPERM IS THE RESULT OF A DYNAMIC COMMUNICATION BETWEEN INTRACELLULAR Ca2+ STORES
Autor/es:
TREVIÑO CLAUDIA L; DE BLAS GERARDO A; MATA MARTINEZ ESPERANZA; MAYORGA LUIS S; ARIAS RODOLFO; DARSZON ALBERTO
Lugar:
Mendoza
Reunión:
Congreso; XXXVI reunión Científica de la SBCuyo; 2018
Institución organizadora:
Sociedad de Biología de Cuyo
Resumen:
Interplay between the signaling pathways of the intracellular second messengers, cAMP and Ca2+, has vital consequences for numerous essential physiological processes in human sperm. This process is necessary for sperm to traverse the egg envelope and to expose a fusogenic membrane that allows egg-sperm fusion. Progesterone and ZP3, the most studied AR inducers, elicit an intracellular Ca2+ increase needed for AR in human sperm. This increase is mediated by a first Ca2+ influx but also the intracellular calcium stores are involved. It is know that intracellular Ca2+ stores play a central role in the regulation of [Ca2+]i and in the generation of complex Ca2+ signals such as oscillations and waves. In a previous report we have proposed that in human sperm the cAMP analogue 8-(p- chlorophenylthio)-2´-O-methyladenosine-3´, 5´-cyclic monophosphate (8-pCPT-2´-O-Me-cAMP) elicits an intracellular Ca2+ release which is involved in AR. The aim of this study was investigate which Ca2+ stores and Ca2+ channels are involved in the 8-pCPT-2´-O-Me-cAMP pathway in human sperm. Performing real time dynamic assays in calcium-free medium -with high time and spatial resolution- and using fluorescent Ca2+ sensors, we found that 8-pCPT-2´-O-Me-cAMP, induce a Ca2+ wave that starts in the posterior neck region and propagates to the acrosome region. This calcium response was sensitive to NED-19 and Thapsigargin, highlighting the participation of ionic channels (Two Pore Channels) and Ca2+ stores (Ca2+-ATPasas: SPCA) on the Ca2+ wave described.