PEDF (Pigment epithelium derived factor) EXPRESSION IN MURINES MALE REPRODUCTIVE SYSTEM

FUNES A.K.; LOPEZ ME; MONCLUS MA; CONTE MI; COLOMBO R; PIETOBON EO; SAEZ LANCELLOTTI T.E.; BARAUNA AA; FORNÉS M.W

Mendoza

Simposio; 2nd Freiburg-Mendoza Symposium on Traslational Medicine; 2019

U.N. de Cuyo- U. de Freiburg- U.B.A.

PEDF (Pigment epithelium derived factor) EXPRESSION IN MURINES MALE REPRODUCTIVE SYSTEMConte MI, Saez Lancelloti E, Funes AK, Colombo RL, Barauna AA, Lopez ME, Pietrobon EO, Fornés MW, Monclus MALaboratorio de Investigaciones Andrológicas. Instituto de Histología y Embriología de Mendoza. CCT CONICET marinesconte@gmail.com Introduction: Pigment Epithelium-Derived Factor also known as Serpin 1F is a glycoprotein from the serine protease inhibitors family broadly extended in many mammal tissues. Their important functions in cell survival and repairmen turn it a source of study for chronic and metabolic disease. Recently, has been described in physiological and pathological situations in the female reproductive system. It`s known their function in normal ovulation where their expression is antagonistic with the vascular endothelium growing factor VEGF. In previous works our group described PEDF expression as androgen-dependent in rat male reproductive system, particularly in epididymis in cauda, but was not found in testes. It has not been described their presence in mouse models yet.Objective: describe the basal expression of PEDF and their antagonist VEGF in male mouse reproductive system. Methods: MEPC5 cells were obtained and immortalized from C57bl/6 mice epididymis and wild type C57Bl/6 tissues were characterized by immunohistochemistry, immunofluorescence and western blot to show the expression of PEDF and VEGF. Androgen receptor (AR) was also analyzed both in the cells in culture and over mice tissues.Results: in MEPC5 cells PEDF and VEGF and AR were detected, demonstrating the usefulness of this cellular model for in vitro studies related to the androgenic dependence in the expression of PEDF and its antagonist VEGF. In mouse tissues we observed PEDF expression over seminiferous epithelium and peritubular cells. This result differs from that observed previously in Wistar rats. In addition, VEGF was detected over seminiferous tubule and Leydig interstitials cells. In epididymal sections PEDF was detected over epithelial cells in a cytoplasmic and nuclear localization in cauda samples and mainly over cytoplasm in caput samples. The intensity of the mark decreased in caudal sense along the organ. VEGF was detected over epithelial cells along the different epididymal regions.