ESSENTIAL OIL OF A. mendozana INHIBITS THE PROLIFERATION OF TUMOR B16F0 CELLS AND INDUCES SENESCENCE

MARRA F; MILLÁN M E ; LIZARRAGA E ; SALVARREDI L ; LÓPEZ LA

Parana, Entre Rios

Congreso; LIVReunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2018

SAIB

The basic pathophysiology of cancer comprises aberrations at different points in the cell cycle. Due to the increasing incidence of cancer worldwide, there is an intense search for new therapeutic strategies to treat this disease. In this area, important efforts have been oriented to exploring the action of compounds of plant origin. Artemisia mendozana var mendozana (ajenjo), is a plant belonging to the Asteraceae family that growth in the piedmont of Mendoza province and it is used as a medicinal plant with antispasmodic and antifungal properties. The essential oil of ajenjo (EAO) contains 28 compounds of which the highest concentrated are: artemisia alcohol 4.8%, α-thujone 5.1%, borneol 11.2% and bornyl acetate 43.7%. In this project the effect of EAO was analyzed in in vitro proliferation of B16F0 murine melanoma cells. For the assay the cells were cultured with vehicle DMSO (control) or 13-45 µg/mL EAO dissolved in DMSO for 72 h. The growth rate (GI) ± SE was calculated from 3 independent experiments. At 72 h of culture, GI of the control was 8.4±0.33 and with EAO was: 5.8±0.56 (A); 3.1±0.03 (B); 2.4±0.47 (C); 2.1±0.20 (D) and 1.5±0.17 (E) for 13,18, 27, 36 and 45 µg/mL respectively. GI of D and E were significantly different to the control (p≤0.001). At 72 h of culture, the percentage of senescent cells of the control was 20.9±1.7 and with EAO 56.5±8.3, 51.2±3.0, 51.1±3.2, 31.8±5 and 35.8±9.6 for 13,18, 27, 36 and 45 µg/mL respectively. These results show that EO, at low concentrations, is a powerful inhibitor of the in vitro proliferation of B16 F0 cells.