Effects of nutrition diets in cadmium environmental intoxication on rat mammary gland response.

SANCHEZ ES; ARNAL N; MARTIN MOLINERO GD; GIMENEZ MS; VÁSQUEZ E; BOLDRINI G; GOMEZ N; MICHEL MC; ALZA NP; PEREZ CHACA MV; ALVAREZ SM

Mar del Plata

Congreso; Reunión Conjunta de la Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Fisiología (SAFIS) 2018; 2018

Sociedad Argentina de Investigación Clínica

Cadmium (Cd) is a toxic element and an important environmental contaminant. We studied the effects of this metal at the molecular, biochemical and cellular levels on rat mammary gland (MG) and looked at the potential protective outcome of a Soy based diet. For this purpose, 4 female lots of Wistar rats were used: 2 lots received casein and 2 lots soybean (Soy) as protein source. Within each group, 1 lot received regular water (control) and the other, 15 ppm of Cd in the drinking water for 60 days (n= 6 animals per group, from three independent experiments). Lipids were extracted by Hexano:Isopropanol:BHT (3v:2v:1%). Total cholesterol, phospholipids and fatty acids were determined by colorimetric assay, thin-layer chromatography and gas chromatography-mass spectrometry, respectively. HMG CoA Reductase (HMGCoAR) and NFκB activation were determined by Western blot. The inflammation marker COX2 and the Bax/Bcl2 ratio were measured by RT-PCR. MGs were subjected to hematoxylin-eosin stain for morphological studies. Our results show that Cd alters the lipid profile of the gland and this effect is modulated by Soy. The expression of HMGCoAR is affected by Cd and Soy (*p