IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
THE ROLE OF MITOCHONDRIA IN CALCIUM SIGNALING ACTIVATED BY PROGESTERONE IN HUMAN SPERM.
Autor/es:
JUSTRIBÓ G; MAYORGA LS.; SOSA CM; ARGUMEDO, M; ARIAS RJ; POBLETE S.; DE BLAS GA
Lugar:
Merlo. San Luis
Reunión:
Congreso; XXXV Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2017
Institución organizadora:
Sociedad de Biología de Cuyo
Resumen:
The main function of mitochondria is the production of ATP through the electron transport chain and lipid oxidation. In addition, this organelle play an important role in Ca2+ buffering and signaling, shaping and extending the kinetics of Ca2+ signals. Calcium signaling is a key regulatory mechanism in sperm functions such as capacitation, motility, hyperactivation, chemotaxis and acrosome reaction. Progesterone (Pg) has been associated with several processes of sperm physiology, since it directly activates membrane Ca2+ channels. Our group in previous work have observed that Pg induces an intracellular calcium increase in media with different [Ca2+], yet is unclear if this calcium increase is due to calcium release from intracellular stores or other source. The aim of this study was to investigate the role of mitochondria in calcium signaling involved in the Pg pathway in human spermatozoa. We used real time dynamic assays with high speed and spatial resolution ? in single cells and population ? and fluorescent calcium sensors. To meet this goal we loaded capacitated human sperm with Fluo3-AM or Fura2-AM, then incubated in medium containing different [Ca2+] and treated with progesterone in absence or presence of mitochondrial modulators. We observed that media with different [Ca2+] generated increases of intracellular Ca2+ with particular kinetics and patterns in response to Pg. We also noticed that mitochondrial modulators altered the Ca2+ patterns and kinetics previously observed. These results suggest that mitochondria participates in calcium signaling in response to Pg.