Soy Protein Modulates the Effects of Intoxication with Cadmiun in Rat Mammary Gland

SANCHEZ ES; ALVAREZ SM; MICHEL MC; VÁSQUEZ E

Merlo

Congreso; XXXV Reunión Anual de la Sociedad de Biología de Cuyo; 2017

We studied the effects of different diets at molecular, biochemical and cellular levels on rat mammary gland (MG). We also looked at the potential protective outcome of those diets under exposure to Cadmiun (Cd), which is a toxic element and an important environmental contaminant. For this purpose, 4 female lots Wistar rats were used: 2 lots received casein and 2 lots soybean (Soy) as protein source. Within each group, 1 lot received regular water (control) and the other, 15 ppm of Cd in the drinking water for 60 days (six animal per group, at least three independent experiments). Lipids were extracted by Hexano:Isopropanol:BHT( 3v:2v:1%.) solvent. Total cholesterol, phospholipids and fatty acids where determined by colorimetric assay, thin-layer chromatography and gas chromatography-mass spectrometry, respectively. HMG CoA Reductase (HMGCoAR) and NFκB activation were determined by Western blot. The inflammation marker COX2 and Bax/Bcl2 were measured by PCR. MGs were subjected to hematoxylin-eosin stain. Our results show that Cd alters the lipid profile of the gland and these effects are modulated by Soy based diet. The expression of HMGCoAR is also affected by Cd and Soy, showing some synergism. On the other hand, both Cd and Soy activate NFκB and this effect is also accompanied by augmented expression of COX2 and apoptosis according to the Bax/Bcl2 relation. Also Cd and Soy stimulate the development but Soy decreases the fat pad. The double treatment cause fibrosis and lost functionality. In summary, Cd affects the fisiology and development of rat MGs and Soy diet may modulates the facts of Cd exposure in this tissue.