IHEM   20887
INSTITUTO DE HISTOLOGIA Y EMBRIOLOGIA DE MENDOZA DR. MARIO H. BURGOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
14-3-3 gamma and beta have opposite roles in adipogenic differentiation.
Autor/es:
ALDANA D. GOJANOVICH; DIEGO M. BUSTOS; MARINA UHART; DIEGO MASONE
Lugar:
Buenos Aires
Reunión:
Congreso; Reunión Conjunta de Sociedades de Biociencia 2017.; 2017
Institución organizadora:
Sociedad Argentina de Investigación Clínica (SAIC)
Resumen:
The14-3-3 protein family regulates several cellular processes includingdifferentiation. Regarding adipogenic differentiation, this familybinds to regulatory proteins involved in adipogenesis as TAZ, PPARγ2,Lipin-1 and Seipin. 14-3-3 proteins bind their targets throughspecific phospho-Serine/Threonine residues. Among the seven highlyconserved mammalian family members, specifically 14-3-3γ and β mRNAand protein levels increase during adipogenic differentiation of3T3-L1 preadipocytes. To investigate the role of these paralogs inadipogenesis, we decreased their levels by using lentiviraltransduction particles containing short hairpin RNAs (shRNAs)specific to silence the expression of both of these paralogs, and theZsGreen protein as a marker of transduced cells. 3T3-L1 cells wereinfected with these vectors, and then induced to the adipogeniclinage during 7 days. Adipogenic differentiation was evaluated by OilRed O staining of lipid droplets followed by confocal microscopyanalysis. The results revealed an increment of number and size oflipids droplets after 14-3-3γ silencing compared to neighboringcells that were not infected. The opposite effect was observed upon14-3-3β silencing, where a pronounced decrease in lipid dropletsnumber and sizes was evident in transduced cells. Taken together ourfindings suggest that 14-3-3γ and β differentially regulate 3T3-L1adipogenesis.